The Science-Backed Peptide Super-App -

Peptide Reconstitution Guide

Use the peptide calculator above to work out accurate dosages - just three easy steps!

Peptide vial strength
Step 1

Enter peptide amount

Put in the total peptide strength (mg) printed on your vial.

Bacteriostatic water volume
Step 2

Enter volume of diluent

Fill in the volume of bacteriostatic water added to the vial (in mL).

Setting the peptide dose
Step 3

Enter desired dose

Input the dose you wish to give per injection in micrograms (mcg).

What is a Peptide Calculator?

A peptide calculator (sometimes called a reconstitution calculator or a peptide dosage calculator) is a tool that converts your vial’s peptide amount and added solvent volume into a usable concentration for a dose. It answers questions like: “How many mcg/mL is my solution after mixing?” and “How many syringe units equal my target dose?”.

Put simply, it combines reconstitution and dosage calculations in one place.

  1. Reconstitution Calculator: Helps you calculate the concentration of the solution after adding a specific volume of diluent to a peptide vial.
  2. Dosage Calculator: Uses the above concentration to tell you exactly how much volume (or syringe units) is needed for your desired dose.

A peptide calculator automates these steps to eliminate manual math errors and to provide precision and consistency. Since peptides are very potent at low doses, even small mistakes can have big effects. Use the Longeviters peptide calculator to dose consistently!

How Does the Peptide Calculator Work?

The peptide reconstitution calculator is simple to use. You just need to choose your peptide, enter the vial amount, the volume of your diluent, and the desired dose. Lastly, pick the size of the syringe. The peptide calculator will then automatically perform the necessary unit conversions and formulae.

As a result, it will display:

  1. Concentration of your reconstituted solution (mcg/mL)
  2. Draw volume (in mL and syringe units) for your target dose
  3. Estimated doses per vial

That should eliminate any potential confusion or manual math - you just draw the number of units in the syringe to get exactly the dose you need. If the calculated volume is larger than the size of your syringe, the tool will clearly showcase that, as well.

Step-by-Step Example

Let's say you have a 5mg peptide vial. You add 3mL of bacteriostatic water to it. You want a 250mcg dose. This is what the Longeviters peptide dosage calculator does:

  1. Concentration: (5 \text{mg} \times 1000 = 5000mcg) total peptide in 3mL, so concentration = 5000mcg/3mL ≈ 1667mcg/mL.
  2. Volume for 250mcg: 250mcg / 1667mcg/mL ≈ 0.15mL.
  3. Syringe units: 0.15mL x 100 = 15 units on a U-100 syringe.

So for a 250mcg dose, you would draw 15 units (0.15mL).

The higher the dilution, the lower the concentration, so you'll need to draw more volume per dose (and vice versa). The calculator deals with this automatically. Always verify your entries against the visual output.

Quick Reference Charts

You can save some time by checking out the charts below for common dosing references and syringe information. Note that they assume the use of U-100 insulin syringes (100 units = 1ml).

Dose-to-Units Reference

Peptide in Vial (mg) Diluent Added (mL) Concentration (mcg/mL) 250 mcg Dose Volume Syringe Units (U-100)
2 mg 1 ml 2000 0.125 mL 13
5 mg 2 ml 2500 0.10 mL 10
10 mg 2 ml 5000 0.05 mL 5
10 mg 3 ml 3333 0.075 mL 7.5
15 mg 3 ml 5000 0.05 mL 5
15 mg 5 ml 3000 0.083 mL 8.3
Table: Example unit dosage reference for 250 mcg.

Example: 5mg vial + 2mL = 2500mcg/mL, so 250mcg = 0.10mL (10 units). Adjust values to your dose, as needed.

Types of Syringes

Syringe Notes
Insulin Syringe (U-100)
0.3 mL (30U)
Fine gradations (0.5–1U) for small doses; 1U = 0.01 mL. Great for <50 mcL draws.
Insulin Syringe (U-100)
0.5 mL (50U)
Mid-range syringe; still 100U/mL. Use when doses are moderate.
Insulin Syringe (U-100)
1.0 mL (100U)
Most common. 1U = 0.01 mL; best for larger volumes.
Tuberculin Syringe 1 mL
(100U)
Same graduations as U-100 (1U = 0.01 mL). Can be used equivalently.
Other Syringes (U-40) Do not use U-40 syringes for peptide dosing. U-40 has 40 units/mL, and applying U-100 math would cause a x2.5 error.
Table: Syringe types explained.

Types of Diluents

Diluent Notes
Bacteriostatic Water Preservative allows multi-dose vials (stable ~28 days refrigerated). Standard choice for most peptide protocols.
Sterile Water No preservative - use only once. Use within 24 hours and discard unused solution.
Normal Saline Usually not recommended for peptides (pH can differ). If used, note that it does not contain a preservative.
Table: Diluent types.

Storage and Reconstitution Guidelines

Whenever you receive a lyophilised (powder) peptide, store the vial refrigerated (2-8 °C) short-term, or frozen (-20 °C) long-term. Upon reconstitution:

  1. With Bacteriostatic Water: Label and refrigerate immediately. The solution is stable for about 28 days. Thaw frozen aliquots as needed, and avoid repeated freeze / thaw cycles.
  2. With Sterile Water: Use within 24 hours, and don’t try to draw multiple times from the vial.

Before reconstitution, ensure that the peptide powder and solvent that were stored in the cold are warmed up to room temperature.

Do not use non-pharmaceutical liquids (tap water, saline with additives, etc.) as they can degrade the peptide quality and introduce contaminants. Use only the recommended sterile diluents.

All information displayed above is for research / educational purposes only and is not a substitute for professional medical advice.

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Why Use Our Peptide Dosage Calculator?

The Longeviters peptide calculator combines peptide reconstitution and dosage calculations into a single, easy-to-use tool.

Extensive Peptide Library

Choose from 30+ popular peptides without manually entering any details. Get calculations tailored specifically to the peptide you're using.

Reconstitution + Dosage

Calculate peptide reconstitution and dosage from a single interface. No need to switch between multiple tools, notes, or spreadsheets.

Single Peptides, Stacks & Blends

Works with individual peptides, peptide stacks, and multi-compound blends. Ideal for more advanced users and high-level protocols.

Flexible Unit Support

Calculate using mcg, mg, and IU measurements depending on your protocol. Easily convert and work with the units relevant to your needs.

FAQ

What is a peptide calculator?

A peptide calculator helps you figure out the concentration and dose volume after you have mixed the peptide powder with water. You enter the vial amount, water volume and desired dose, and it tells you how many mL or syringe units to draw.

What is the best tool to calculate peptide dosage?

Peptides AI - the app by Longeviters - comes equipped with the best peptides calculator and reconstitution tool on the market today. It’s informative, very simple to use, and is integrated into a larger peptide-oriented ecosystem. You can calculate your doses for different peptides, add them to your stack, track your progress, and do so much more!

How to convert mg to mcg and syringe units?

1mg is equal to 1,000mcg. Using a U-100 insulin syringe, 100 units = 1mL, therefore - 1 unit = 0.01mL. For example, 0.1mL = 10 units, 0.05 = 5 units. The Longeviters peptide reconstitution calculator will tell you the dose in mL and units.

How do I prepare my peptide?

It depends on how concentrated you want it to be. The standard practice is 1-3 mL per 5-10 mg vial. More water = lower concentration (less mcg/mL), meaning you will need to draw a larger volume per dose. Use the Longeviters peptide calculator - enter a trial volume, and it will show how many units your target dose will be. Adjust the volume until your dose falls in a convenient range.

Are the results from the calculator valid for any syringe?

No. The peptide reconstitution calculator assumes a U-100 insulin syringe (100 units = 1mL). Do not use veterinary (U-40) syringes - they are 40 units/mL. If you did U-100 math on a U-40 syringe, that would be a 2.5x overdose. For very small volumes (<10 units) use a 0.3mL (30U) insulin syringe for better accuracy.

Is the peptide calculator free to use?

Yes, the peptide reconstitution calculator online is free to use, and should be used for informational / research purposes. Keep in mind that it’s not a substitute for professional medical advice. Always follow the instructions and regulatory guidelines of your supplier when handling peptides. If you want a more thorough tool to elevate your peptide use, do also check out Peptides AI - the mega app for all things peptides, including dosage and reconstitution calculators.

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